Presence of low concentrations (1–2%) of ethanol during irradiation exhibited significant protection against DNA damagecaused by very high doses (2–12 kGy) of 60Co-gamma-rays in vitro. Radiation-induced DNA damage was substantiallyreduced in different types of DNA molecules (chromosomal DNA from Anabaena 7120 or Deinococcus radiodurans orbacteriophage Lambda, and plasmid pBluescript DNA) when irradiated in the presence of ethanol, thus indicating thegeneric nature of ethanol protection. The radioprotection appeared to be a consequence of the well known ability of ethanolto scavenge hydroxyl radicals. Addition of ethanol during 6 kGy irradiation also reduced DNA damage in vivo andimproved post-irradiation growth recovery of Anabaena 7120 cultures. To our knowledge, this is the first instance of abilityof very low ethanol concentrations to protect DNA from damage triggered by extremely high doses of 60Co-gamma rays.
Volume 46, 2020
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