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      https://www.ias.ac.in/article/fulltext/jbsc/042/04/0657-0664

    • Keywords

       

      Anhydrocochlioquinone; Cochliobolus sp.; leishmanicidal; antimicrobial; tumour cells

    • Abstract

       

      The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidalactivity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compoundwere in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC50 value of22.4 lg/mL (44 lM) and, when submitted to the microdilution assay against Gram-positive and Gram-negative bacteria,showed a minimal inhibitory concentration against Staphylococcus aureus ATCC 25295 of 128 lg/mL (248.7 lM). It wasalso active against five human cancer cell lines, showing IC50 values from 5.4 to 20.3 lM. ANDC-A demonstrated adifferential selectivity for HL-60 (SI 5.5) and THP-1 (SI 4.3) cell lines in comparison with Vero cells and was moreselective than cisplatin and doxorubicin against MCF-7 cell line in comparison with human peripheral blood mononuclearcells. ANDC-A was able to eradicate clonogenic tumour cells at concentrations of 20 and 50 lM and induced apoptosis inall tumour cell lines at 20 lM. These results suggest that ANDC-A might be used as a biochemical tool in the study oftumour cells biochemistry as well as an anticancer agent with durable effects on tumours.

    • Author Affiliations

       

      FERNANDA F CAMPOS1 2 JONAS P RAMOS3 DJALMA M DE OLIVEIRA4 TAˆ NIA M A ALVES2 ELAINE M DE SOUZA-FAGUNDES3 CARLOS L ZANI2 FA´ BIO C SAMPAIO5 ATTILIO CONVERTI6 BETANIA B COTA2

      1. Faculty of Medicine, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, Brazil
      2. Laboratory of Chemistry of Bioactive Natural Products, Centro de Pesquisas Rene´ Rachou, Fundac¸a˜o Oswaldo Cruz, Rio de Janeiro, Brazil
      3. Department of Physiology and Biofisics, Federal University of Minas Gerais, Belo Horizonte, Brazil
      4. Department of Chemistry, Southwest Bahia State University, Jequie´, Bahia, Brazil
      5. Department of Pharmacy, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, Brazil
      6. Department of Civil, Chemical and Environmental Engineering, University of Genova, Genova, Italy
    • Dates

       
  • Journal of Biosciences | News

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