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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/042/02/0231-0244

    • Keywords

       

      Coronavirus spike protein trafficking; cytoplasmic tail signal; endoplasmic reticulum–Golgi intermediate complex; lysosome

    • Abstract

       

      Intracellular trafficking and localization studies of spike protein from SARS and OC43 showed that SARS spikeprotein is localized in the ER or ERGIC compartment and OC43 spike protein is predominantly localized in thelysosome. Differential localization can be explained by signal sequence. The sequence alignment using Clustal Wshows that the signal sequence present at the cytoplasmic tail plays an important role in spike protein localization. Aunique GYQEL motif is identified at the cytoplasmic terminal of OC43 spike protein which helps in localization in thelysosome, and a novel KLHYT motif is identified in the cytoplasmic tail of SARS spike protein which helps in ER orERGIC localization. This study sheds some light on the role of cytoplasmic tail of spike protein in cell-to-cell fusion,coronavirus host cell fusion and subsequent pathogenicity.

    • Author Affiliations

       

      JIBIN SADASIVAN1 MANMEET SINGH1 JAYASRI DAS SARMA1

      1. Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur 741 246, India
    • Dates

       
  • Journal of Biosciences | News

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