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      https://www.ias.ac.in/article/fulltext/jbsc/042/01/0131-0138

    • Keywords

       

      Food intake; hypothalamus; neonatal maternal separation; stress

    • Abstract

       

      The aim of this study was to investigate whether neonatal maternal separation (MS) – chronic stress experience inearly life – affects the anorectic efficacy of leptin in the offspring at adolescence. Sprague–Dawley pups wereseparated from the dam daily for 3 h during postnatal day 1–14 or left undisturbed as non-handled controls (NH).NH and MS male pups received an intraperitoneal leptin (100 μg/kg) or saline on postnatal day (PND) 28, and thenfood intake and body weight gain were recorded. The hypothalamic levels of leptin-signalling-related genes,phosphorylated signal transducer and activator of transcription-3 (pSTAT3) and protein-tyrosine phosphatase 1B(PTP1B) were examined at 40 min after a single injection of leptin on PND 39 by immunohistochemistry and Westernblot analysis. Leptin-induced suppressions in food intake and weight gain was observed in NH pups, but not in MS.Leptin increased pSTAT3 in the hypothalamic arcuate nucleus of NH pups, but not of MS. Interestingly, basal levelsof the hypothalamic PTP1B and pSTAT3 were increased in MS pups compared with NH controls. The results suggestthat neonatal MS experience may blunt the anorectic efficacy of leptin later in life, possibly in relation with increasedexpressions of PTP1B and/or pSTAT3 in the hypothalamus.

    • Author Affiliations

       

      JH LEE1 SB YOO1 JY KIM1 JY LEE1 BT KIM2 K PARK3 JW JAHNG1

      1. Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, Seoul, 110-768, Korea
      2. Department of Family Practice and Community Health, Ajou University School of Medicine, Suwon, 443-721, Korea
      3. Department of Physiology Dental Research Institute, Seoul National University School of Dentistry,
    • Dates

       
  • Journal of Biosciences | News

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