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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/042/01/0103-0111

    • Keywords

       

      Cell proliferation; hepatocellular carcinoma; invasion; migration; miR-144; ZFX

    • Abstract

       

      MicroRNA 144 (miR-144), a small non-coding RNA, is frequently dysregulated in human several tumour progression,but its role and the underlying mechanisms in hepatocellular carcinoma (HCC) is poorly investigated. In thepresent study, the expression of miR-144 was firstly analysed in datasets derived from GSE21362 and TCGA, andthen detected in HCC tissues and cell lines by quantitative RT-PCR (qRT-PCR) analysis. MiR-144 was shown to besignificantly down-regulated in HCC tissues and cell lines. Subsequently, overexpression of miR-144 was transfectedinto HCC cell lines so as to investigate its biological function, including MTT, colony formation, and transwell assays.Gain of function assay revealed miR-144 remarkably inhibited cell proliferation, migration and invasion. In addition,bioinformatical analysis and luciferase reporter assay identified ZFX as a novel target of miR-144 in HCC cells, asconfirmed by qRT-PCR and Western blot. Furthermore, ZFX was found to be significantly up-regulated usingOncomine database analysis. Loss of function assay further indicated knockdown of ZFX had similar effects ofmiR-144-mediated HCC cell proliferation and invasion. Therefore, miR-144 has been demonstrated to act as a tumoursuppressor in HCC cell growth and motility by directly targeting ZFX, which implicates its potential applications inthe development of HCC treatment.

    • Author Affiliations

       

      HONGBIN BAO1 XINGUO LI1 HENGLI LI1 HONGLI XING1 BINGHUI XU1 XIANFENG ZHANG1 ZHAOMING LIU1

      1. Department of Hepatobiliary Surgery, Harrison International Peace Hospital, Hengshui City, Hebei province, PR of China
    • Dates

       
  • Journal of Biosciences | News

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