• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/042/01/0023-0030

    • Keywords

       

      Apoptosis; hsa-miR-939; luciferase assay; neurotrophin signalling; NGFR

    • Abstract

       

      Neurotrophin receptors play a crucial role in neuronal survival, differentiation and regeneration. Nerve growthfactor receptor (NGFR) or P75NTR is a neurotrophin receptor that is involved in many pathological conditionsincluding cancers. Genetic factors that are involved in regulation of neurotrophin receptors are under intenseinvestigation. MiRNAs are novel regulators of signalling pathways that are candidates for regulation ofneurotrophin receptors. Computational programs predicted that NGFR gene is a bona fide target for hsa-miR-939. RT-qPCR, Western analysis and dual luciferase assay evidences indicated that NGFR transcript is targetedby hsa-miR-939. Also, hsa-miR-939 overexpression brought about down-regulation of NGFR expression in U87cell line, followed by cell death rate reduction, detected by flow cytometry. Taken together, here for the first time,hsa-miR-939 is introduced as a novel key regulator of NGFR expression and its involvement in cell death/survivalprocesses is suggested.

    • Author Affiliations

       

      FAHIMEH HOSSEINI AGHDAEI1 BAHRAM M SOLTANI1 SADAT DOKANEHIIFARD1 SEYED JAVAD MOWLA1 MASOUD SOLEIMANI2

      1. Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
      2. and 2Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.