DWF4 and CPD are key brassinosteroids (BRs) biosynthesis enzyme genes. To explore the function of Populuseuphratica DWF4 (PeDWF4) and CPD (PeCPD), Arabidopsis thaliana transgenic lines (TLs) expressing PeDWF4,PeCPD or PeDWF4 plus PeCPD, namely PeDWF4-TL, PeCPD-TL and PeCP/DW-TL, were characterized. Comparedwith wild type (WT), the changes of both PeDWF4-TL and PeCPD-TL in plant heights, silique and hypocotylslengths and seed yields were similar, but in bolting time and stem diameters, they were opposite. PeCP/DW-TL wasmore in plant heights and the lengths of primary root, silique, and fruit stalk, but less in silique numbers and seedyields than either PeDWF4-TL or PeCPD-TL. PeDWF4 and PeCPD specially expressed in PeDWF4-TL or PeCPDTL,and the transcription level of PeDWF4 was higher than that of PeCPD. In PeCP/DW-TL, their expressions wereall relatively reduced. Additionally, the expression of PeDWF4 and PeCPD differentially made the expression levelsof AtDWF4, AtCPD, AtBR6OX2, AtFLC, AtTCP1 and AtGA5 change in the TLs. The total BRs contents werePeDWF4-TL > PeCP/DW-TL > WT > PeCPD-TL. These results imply that PeDWF4 is functionally not exactly thesame as PeCPD and there may be a synergistic and antagonistic effects in physiology between both of them in theregulation of plant growth and development.
Volume 45, 2020
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