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    • Keywords


      Contractile vacuole; DdRab2; DdRabS; Dictyostelium discoideum; Golgi

    • Abstract


      Small-molecular-weight GTPase Rab2 has been shown to be a resident of pre-Golgi intermediates and is required for protein transport from the ER to the Golgi complex; however, Rab2 has yet to be characterized in Dictyostelium discoideum. DdRabS is a Dictyostelium Rab that is 80% homologous to DdRab1 which is required for protein transport between the ER and Golgi. Expression of GFP-tagged DdRab2 and DdRabS proteins showed localization to Golgi membranes and to the contractile vacuole system (CV) in Dictyostelium. Microscopic imaging indicates that the DdRab2 and DdRabS proteins localize at, and are essential for, the proper structure of Golgi membranes and the CV system. Dominant negative (DN) forms show fractionation of Golgi membranes, supporting their role in the structure and function of it. DdRab2 and DdRabS proteins, and their dominant negative and constitutively active (CA) forms, affect osmoregulation of the cells, possibly by the influx and discharge of fluids, which suggests a role in the function of the CV system. This is the first evidence of GTPases being localized to both Golgi membranes and the CV system in Dictyostelium.

    • Author Affiliations


      Katherine Maringer1 2 3 Azure Yarbrough3 Sunder Sims-Lucas2 Entsar Saheb3 4 Sanaa Jawed3 4 John Bush3

      1. Division of Nephrology, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh PA 15201, USA
      2. Rangos Research Center, Children’s Hospital of Pittsburgh of UPMC, Pittsburgh PA 15224, USA
      3. Biology Department, University of Arkansas at Little Rock, Little Rock, AR 72204-1099, USA
      4. Biology Department, Baghdad University, Iraq
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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