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      https://www.ias.ac.in/article/fulltext/jbsc/041/02/0173-0182

    • Keywords

       

      DNA replication; HCMV; Snapin; UL130

    • Abstract

       

      The interplay between the host and Human cytomegalovirus (HCMV) plays a pivotal role in the outcome of an infection. HCMV growth in endothelial and epithelial cells requires expression of viral proteins UL128, UL130, and UL131 proteins (UL128-131), of which UL130 is the largest gene and the only one that is not interrupted by introns. Mutation of the C terminus of the UL130 protein causes reduced tropism of endothelial cells (EC). However, very few host factors have been identified that interact with the UL130 protein. In this study, HCMV UL130 protein was shown to directly interact with the human protein Snapin in human embryonic kidney HEK293 cells by Yeast two-hybrid screening, in vitro glutathione S-transferase (GST) pull-down, and co-immunoprecipitation. Additionally, heterologous expression of protein UL130 revealed co-localization with Snapin in the cell membrane and cytoplasm of HEK293 cells using fluorescence confocal microscopy. Furthermore, decreasing the level of Snapin via specific small interfering RNAs decreased the number of viral DNA copies and titer in HCMV-infected U373-S cells. Taken together, these results suggest that Snapin, the pUL130 interacting protein, has a role in modulating HCMV DNA synthesis.

    • Author Affiliations

       

      Guili Wang1 2 Gaowei Ren1 Xin Cui1 Yanpin Ma1 Ying Qi1 Yujing Huang1 Zhongyang Liu1 Zhengrong Sun1 Qiang Ruan1

      1. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China
      2. Department of Pediatrics, The Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, Liaoning, China
    • Dates

       
  • Journal of Biosciences | News

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