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      https://www.ias.ac.in/article/fulltext/jbsc/040/05/0845-0853

    • Keywords

       

      ARS element; autonomous replication; late replication enforcing element (LRE); replication origin; Schizosaccharomyces pombe

    • Abstract

       

      Short, specific DNA sequences called as Autonomously Replicating Sequence (ARS) elements function as plasmid as well as chromosomal replication origins in yeasts. As compared to ARSs, different chromosomal origins vary greatly in their efficiency and timing of replication probably due to their wider chromosomal context. The two Schizosaccharomyces pombe ARS elements, ars727 and ars2OO4, represent two extremities in their chromosomal origin activity - ars727 is inactive and late replicating, while ars2OO4 is a highly active, early-firing origin. To determine the effect of chromosomal context on the activity of these ARS elements, we have cloned them with their extended chromosomal context as well as in the context of each other in both orientations and analysed their replication efficiency by ARS and plasmid stability assays. We found that these ARS elements retain their origin activity in their extended/altered context. However, deletion of a 133-bp region of the previously reported ars727-associated late replication enforcing element (LRE) caused advancement in replication timing of the resulting plasmid. These results confirm the role of LRE in directing plasmid replication timing and suggest that the plasmid origin efficiency of ars2OO4 or ars727 remains unaltered by the extended chromosomal context.

    • Author Affiliations

       

      Aditya S Pratihar1 Vishnu P Tripathi2 Mukesh P Yadav3 Dharani D Dubey2

      1. Department of Biotechnology, Dayanand Academy of Management Studies, Govlnd Nagar, Kanpur, U.P. 208006, India
      2. Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur 222003, U.P., India
      3. E-407, Centre for Cellular & Molecular Biology, Uppal Road Hubslguda, Hyderabad Telangana 500007, India
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    • Supplementary Material

       
  • Journal of Biosciences | News

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