Porcine circovirus type 2 ORF4 protein binds heavy chain ferritin
Qizhuang Lv Kangkang Guo Tao Wang Chengcheng Zhang Yanming Zhang
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Porcine circovirus type 2 (PCV2) is the primary infectious agent of PCV-associated disease (PCVAD) in swine. ORF4 protein is a newly identified viral protein of PCV2 and is involved in virus-induced apoptosis. However, the molecular mechanisms of ORF4 protein regulation of apoptosis remain unclear, especially given there is no information regarding any cellular partners of the ORF4 protein. Here, we have utilized the yeast two-hybrid assay and identified four host proteins (FHC, SNRPN, COX8A and Lamin C) interacting with the ORF4 protein. Specially, FHC was chosen for further characterization due to its important role in apoptosis. GST pull-down, subcellular co-location and co-immunoprecipitation assays confirmed that the PCV2 ORF4 protein indeed interacted with the heavy-chain ferritin, which is an interesting clue that will allow us to determine the role of the ORF4 protein in apoptosis.
Qizhuang Lv1 Kangkang Guo1 Tao Wang1 Chengcheng Zhang1 Yanming Zhang1
Volume 48, 2023
Continuous Article Publishing mode
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