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      https://www.ias.ac.in/article/fulltext/jbsc/040/02/0233-0240

    • Keywords

       

      Avian influenza virus; NP; PB2; siRNA; subtype H5N1

    • Abstract

       

      Highly Pathogenic Avian Influenza (HPAI) H5N1 virus is a threat to animal and public health worldwide. Till date, the H5N1 virus has claimed 402 human lives, with a mortality rate of 58% and has caused the death or culling of millions of poultry since 2003. In this study, we have designed three siRNAs (PB2-2235, PB2-479 and NP-865) targeting PB2 and NP genes of avian influenza virus and evaluated their potential, measured by hemagglutination (HA), plaque reduction and Real time RT-PCR assay, in inhibiting H5N1 virus (A/chicken/Navapur/7972/2006) replication in MDCK cells. The siRNAs caused 8- to 16-fold reduction in virus HA titers at 24 h after challenged with 100TCID50 of virus. Among these siRNAs, PB2-2235 offered the highest inhibition of virus replication with 16-fold reduction in virus HA titer, 80% reduction in viral plaque counts and 94% inhibition in expression of specific RNA at 24 h. The other two siRNAs had 68–73% and 87–88% reduction in viral plaque counts and RNA copy number, respectively. The effect of siRNA on H5N1 virus replication continued till 48h (maximum observation period). These findings suggest that PB2-2235 could efficiently inhibit HPAI H5N1 virus replication.

    • Author Affiliations

       

      Padmanava Behera1 Shanmugasundaram Nagarajan1 Harshad V Murugkar1 Semmannan Kalaiyarasu1 Anil Prakash2 Ragini Gothalwal3 Shiv Chandra Dubey1 Diwakar D Kulkarni1 Chakradhar Tosh1

      1. ICAR-National Institute of High Security Animal Diseases, Bhopal 462 022, India
      2. Department of Microbiology, Barkatullah University, Bhopal 462 026, India
      3. Department of Biotechnology, Barkatullah University, Bhopal 462 026, India
    • Dates

       
  • Journal of Biosciences | News

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