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      https://www.ias.ac.in/article/fulltext/jbsc/040/01/0125-0135

    • Keywords

       

      Basic antiviral glycoprotein; BDP-30; Boerhaavia diffusa; induced systemic resistance; ribosome-inactivating proteins

    • Abstract

       

      Root extract of Boerhaavia diffusa L. induced systemic resistance in tobacco against Tobacco mosaic virus. A 30 kDa protein was isolated as the active component, called BDP-30 on the basis of the molecular weight and source plant. BDP-30, a glycoprotein, was found to be temperature and protease resistant. It was basic, possessing a pI greater than 9.0. In-gel proteolytic digestion of BDP-30 generated two peptides that possessed the amino acid sequence KLYDIPPLR and KVTLPYSGNYER by LC/MS/MS. Both peptides shared absolute sequence identity with trichosanthin, a ribosome-inactivating protein from Trichosanthes kirilowii, and a 78% and 100% homology respectively with an RIP from Bryonia dioica, bryodin. Further, effort was made to look at the fate of TMV in induced resistant Nicotiana tabacum cv. Xanthi, a systemic host of the virus, at specified days after inoculation in control and treated plants. TMV coat protein (CP) was detected by immunoblot 7 days post inoculation up to 21 days in the control set, but not in treated resistant plants. TMV RNA was detected by RT-PCR using TMV-CP specific primers. Resistant tobacco did not show presence of TMV RNA up to 21 days of inoculation. This suggests that BDP-30 may be suppressing TMV replication.

    • Author Affiliations

       

      Shalini Srivastava1 H N Verma1 2 Aparana Srivastava1 Vivek Prasad1

      1. Molecular Plant Virology Lab, Department of Botany, Lucknow University, Lucknow 226 007, India
      2. Jaipur National University, Jagatpura, Jaipur 302 017, India
    • Dates

       
  • Journal of Biosciences | News

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