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    • Keywords


      Anti-TNF-𝛼; collagen I; interleukin-12; IRF4; maturation; monocyte-derived dendritic cells

    • Abstract


      Previously we have shown that collagen I enhances the maturation and function of dendritic cells (DCs). Inflammatory mediators such as tumour necrosis factor (TNF)-𝛼, interleukin (IL)-1𝛽 and lipopolysaccharide (LPS) are also known to activate DCs. Here we investigated the involvement of TNF-𝛼 on the collagen I-induced DCs activation. TNF-a neutralization inhibited collagen I-induced IL-12 secretions by DCs. Additionally, we observed suppression of collagen I-induced costimulatory molecules expression along with down-regulation of genes involved in DCs activation pathway. Furthermore, TNF-𝛼 inhibition upon collagen Istimulation up-regulated the expression of interferon regulatory transcription factor IRF4, when compared to collagen I only treated cells. Collectively, our data demonstrate that collagen I induce TNF-𝛼 production, which is crucial for the activation and function of DCs, through down-regulation of IRF4, and implicates the importance in development of anti- TNF-𝛼 therapeutics for several inflammatory diseases.

    • Author Affiliations


      Barun Poudel1 2 Hyeon-Hui Ki1 Young-Mi Lee2 Dae-Ki Kim1

      1. Department of Immunology and Institute of Medical Sciences, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Korea
      2. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Korean Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570-749, Korea
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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