Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells
Goran Petrovski Kata Pásztor László Orosz Réka Albert Edina Mencel Morten C Moe Kai Kaarniranta Andrea Facskó Klára Megyeri
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Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and - 2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1- and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.
Goran Petrovski1 2 Kata Pásztor3 László Orosz3 Réka Albert1 2 Edina Mencel3 Morten C Moe4 Kai Kaarniranta5 Andrea Facskó6 Klára Megyeri3
Volume 48, 2023
Continuous Article Publishing mode
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