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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/039/04/0683-0692

    • Keywords

       

      Apoptosis; autophagy; corneal cells; Herpes simplex virus; keratitis

    • Abstract

       

      Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and - 2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1- and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.

    • Author Affiliations

       

      Goran Petrovski1 2 Kata Pásztor3 László Orosz3 Réka Albert1 2 Edina Mencel3 Morten C Moe4 Kai Kaarniranta5 Andrea Facskó6 Klára Megyeri3

      1. Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
      2. Stem Cells and Eye Research Laboratory and Apoptosis and Genomics Research Group of Hungarian Academy of Sciences, Department of Biochemistry and Molecular Biology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
      3. Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary
      4. Department of Ophthalmology, Center of Eye Research, Oslo University Hospital, University of Oslo, Oslo, Norway
      5. Department of Ophthalmology, Institute of Clinical Medicine, University of Eastern Finland, and Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland
      6. Department of Ophthalmology, University of Szeged, Szeged, Hungary

    • Dates

       
      Published
      September 2014
      Manuscript received
      24 June 2013
      Accepted
      25 May 2014

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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