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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/039/03/0513-0517

    • Keywords

       

      Chickpea; domestication; nitrogen fixation; rhizobia; symbiosis

    • Abstract

       

      Crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (Cicer arietinum) as compared with wild species (C. reticulatum, C. bijugum). To explore impact of domestication on symbiosis, 10 accessions of chickpeas, including 4 accessions of C. arietinum, and 3 accessions of each of C. reticulatum and C. bijugum species, were selected and DNAs were extracted from their nodules. To distinguish chickpea symbiont, preliminary sequences analysis was attempted with 9 genes (16S rRNA, atpD, dnaJ, glnA, gyrB, nifH, nifK, nodD and recA) of which 3 genes (gyrB, nifK and nodD) were selected based on sufficient sequence diversity for further phylogenetic analysis. Phylogenetic analysis and sequence diversity for 3 genes demonstrated that sequences from C. reticulatum were more diverse. Nodule occupancy by dominant symbiont also indicated that C. reticulatum (60%) could have more various symbionts than cultivated chickpea (80%). The study demonstrated that wild chickpeas (C. reticulatum) could be used for selecting more diverse symbionts in the field conditions and it implies that chickpea domestication affected symbiosis negatively in addition to reducing genetic diversity.

    • Author Affiliations

       

      Dong Hyun Kim1 Mayank Kaashyap1 Abhishek Rathore1 Roma R Das1 Swathi Parupalli1 Hari D Upadhyaya1 S Gopalakrishnan1 Pooran M Gaur1 Sarvjeet Singh2 Jagmeet Kaur2 Mohammad Yasin3 Rajeev K Varshney1 4

      1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, India
      2. Punjab Agricultural University, Ludhiana 141 027, India
      3. RAK College of Agriculture, Sehore 466 001, India
      4. CGIAR Generation Challenge Program, C/o CIMMYT, Mexico, DF 06600, Mexico

    • Dates

       
      Published
      June 2014
      Manuscript received
      16 December 2013
      Accepted
      18 March 2014

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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