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    • Keywords


      3-D tomography; Alzheimer's disease; amyloid 𝛽; HVEM; mitochondria

    • Abstract


      Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. The deterioration of subcellular organelles, including the mitochondria, is another major ultrastructural characteristic of AD pathogenesis, in addition to amyloid plaque deposition. However, the three-dimensional (3-D) study of mitochondrial structural alteration in AD remains poorly understood. Therefore, ultrastructural analysis, 3-D electron tomography, and immunogold electron microscopy were performed in the present study to clarify the abnormal structural alterations in mitochondria caused by the progression of AD in APP/PSEN1 transgenic mice, expressing human amyloid precursor protein, as a model for AD. Amyloid 𝛽 (A𝛽) plaques accumulated and dystrophic neurites (DN) developed in the hippocampus of transgenic AD mouse brains. We also identified the loss of peroxiredoxin 3, an endogenous cytoprotective antioxidant enzyme and the accumulation of A𝛽 in the hippocampal mitochondria of transgenic mice, which differs from those in age-matched wild-type mice. The mitochondria in A𝛽 plaque-detected regions were severely disrupted, and the patterns of ultrastructural abnormalities were classified into three groups: disappearance of cristae, swelling of cristae, and bulging of the outer membrane. These results demonstrated that morpho-functional alterations of mitochondria and AD progression are closely associated and may be beneficial in investigating the function of mitochondria in AD pathogenesis.

    • Author Affiliations


      Ki Ju Choi1 2 3 Mi Jeong Kim1 3 A Reum Je1 Sangmi Jun1 Chulhyun Lee4 Eunji Lee5 Mijung Jo5 Yang Hoon Huh1 Hee-Seok Kweon1

      1. Division of Electron Microscopic Research, Korea Basic Science Institute, 169-148 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea
      2. Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, 187 Osong Sangmyeong 2-ro, Gangoe-myeon, Cheongwon-gun, Chungbuk, 363- 951, Korea
      3. These authors contributed equally to this work.
      4. Division of Magnetic Resonance Research, Korea Basic Science Institute, 804-1 Ochang, Chungbuk 363-883, Korea
      5. Graduate School of Analytical Science and Technology, Chungnam National University, 79 Daehangno, Daejeon 305-764, Korea
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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