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https://www.ias.ac.in/article/fulltext/jbsc/038/05/0917-0924

ATP synthase 𝛽 subunit; HSP70; virus–host interaction, virus overlay protein binding assay

White Spot Syndrome Virus (WSSV) is a major pathogen in shrimp aquaculture, and its rampant spread has resulted in great economic loss. Identification of host cellular proteins interacting with WSSV will help in unravelling the repertoire of host proteins involved in WSSV infection. In this study, we have employed one-dimensional and two-dimension virus overlay protein binding assay (VOPBA) followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the host proteins of Penaeus monodon that could interact with WSSV. The VOPBA results suggest that WSSV interacted with housekeeping proteins such as heat shock protein 70, ATP synthase subunit 𝛽, phosphopyruvate hydratase, allergen Pen m 2, glyceraldehyde-3-phosphate dehydrogenase, sarcoplasmic calcium-binding protein, actin and 14-3-3-like protein. Our findings suggest that WSSV exploits an array of housekeeping proteins for its transmission and propagation in P. monodon.

Vinayak Biradar^{1 2} Santosh Narwade^{1 2} Mandar Paingankar^{1 3} Deepti Deobagkar^{1 4}

1. Molecular Biology Research Laboratory, Department of Zoology, Centre for Advance Studies, University of Pune, Pune 411 007, India
2. These authors contributed equally to this work.
3. National Institute of Virology, Microbial Containment Complex, 130/1, Pashan Sus Road( Pashan Pune 411 021, India
4. Bioinformatics Center, University of Pune, Pune 411 007, India

Published

December 2013

Manuscript received

8 April 2013

Accepted

30 August 2013