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      https://www.ias.ac.in/article/fulltext/jbsc/038/05/0899-0904

    • Keywords

       

      LY96; Ka/Ks; positive selection; TLR4; vertebrates

    • Abstract

       

      As a secreted glycoprotein that binds to the extracellular domain of Toll-like receptor 4 (TLR4), Lymphocyte Antigen 96 (LY96), also called myeloid differentiation 2 (MD2), is required for the activation of TLR4 by lipopolysaccharide (LPS) and plays an important role in innate immunity, which is the first line of defence against microbial infections. Previous studies have proposed that mammalian toll-like receptors (TLRs) have evolved under diversifying selection due to their role in pathogen detection. Given the fact that LY96 is highly functionally linked to TLR4, it would be interesting to test whether LY96 is under the intense pressure of natural selection. To investigate the natural selection hypothesis, we compared the coding sequences from 13 vertebrates and evaluated the molecular evolution of LY96 gene in these species. Result shows that natural selection at exon 4 has indeed played a role in shaping the function of LY96 in the course of evolution. In addition to the study of Nakajima, we found the two branch nodes with Ka/Ks ratios greater than 1: the one leading to cow and pig and the other to rabbit and the primates.

    • Author Affiliations

       

      Tonghai Dou1 2 Maobin Fu1 2 Yixia Wang1 Yang Zhao1 Zhengshi Wang3 Zhengqian Bian3 Yan Zhou1 4

      1. Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China
      2. These authors contributed equally to this work.
      3. Department of General Surgery, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200127, People's Republic of China
      4. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, People's Republic of China
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    • Supplementary Material

       
  • Journal of Biosciences | News

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