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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/03/0479-0485

    • Keywords

       

      Down-regulation; HCMV; hcmv-miR-UL36; UL138

    • Abstract

       

      MicroRNAs (miRNAs) are small RNAs, 19–23 nucleotides in length, which regulate a variety of cellular processes. Human cytomegalovirus (HCMV) encodes only one intronic miRNA: human cytomegalovirus microRNA UL36 (hcmv-miR-UL36). In this study, we found that over-expression of hcmv-miR-UL36 resulted in a more than threefold increase in HCMV DNA synthesis at 24 h post infection. Fifteen putative targets of hcmv-miR-UL36 were identified using hybrid PCR, one being the HCMV UL138 gene that has previously been identified as a novel latency-associated determinant of HCMV infection. Down-regulation of UL138 expression by hcmv-miR-UL36 was validated using luciferase reporter assays and Western blot analysis in HEK293 cells. In the presence of hcmv-miR-UL36, we observed a 74.6% decrease in luciferase activity and a 46.2% decrease in HCMV UL138 protein expression. Our results indicate that hcmv-miR-UL36 may be a viral miRNA contributing to HCMV replication.

    • Author Affiliations

       

      Yujing Huang1 Ying Qi2 Yanping Ma2 Rong He1 Yaohua Ji2 Zhengrong Sun2 Qiang Ruan2

      1. Clinical Genetics Department, the Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning, P.R. China
      2. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning, P.R. China
    • Dates

       
  • Journal of Biosciences | News

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