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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/02/0397-0408

    • Keywords

       

      Dot-blot immunoassay; egg paint binder; cultural heritage; ovalbumin; proteomics; pigments

    • Abstract

       

      A protocol for a simple and reliable dot-blot immunoassay was developed and optimized to test work of art samples for the presence of specific proteinaceus material (i.e. ovalbumin-based). The analytical protocol has been extensively set up with respect, among the other, to protein extraction conditions, to densitometric analysis and to the colorimetric reaction conditions. Feasibility evaluation demonstrated that a commercial scanner and a free image analysis software can be used for the data acquisition and elaboration, thus facilitating the application of the proposed protocol to commonly equipped laboratories and to laboratories of museums and conservation centres. The introduction of method of standard additions in the analysis of fresh and artificially aged laboratory-prepared samples, containing egg white and various pigments, allowed us to evaluate the matrix effect and the effect of sample aging and to generate threshold density values useful for the detection of ovalbumin in samples from ancient works of art. The efficacy of the developed dot-blot immunoassay was proved testing microsamples from 13th–16th century mural paintings of Saint Francesco Church in Lodi (Italy). Despite the aging, the altered conditions of conservation, the complex matrix, and the micro-size of samples, the presence of ovalbumin was detected in all those mural painting samples where mass-spectrometry-based proteomic analysis unambiguously detected ovalbumin peptides.

    • Author Affiliations

       

      Michela Gambino1 Francesca Cappitelli1 Cristina Cattò1 Aristodemo Carpen1 Pamela Principi1 2 Lisa Ghezzi3 Ilaria Bonaduce3 Eugenio Galano4 Pietro Pucci4 Leila Birolo4 Federica Villa1 Fabio Forlani1

      1. Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente, Università degli Studi di Milano, Milano, Italy
      2. Bio & Environmental Technologies, Institute CIM for Sustainable Innovation, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Manno, Switzerland
      3. Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Pisa, Italy
      4. Dipartimento di Scienze Chimiche, Università di Napoli “Federico II”, Napoli, Italy

    • Dates

       
      Published
      June 2013
      Manuscript received
      19 November 2012
      Accepted
      11 March 2013

    • Supplementary Material

       

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

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