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    • Development of rapid phenotypic system for the identification of Gram-negative oxidase-positive bacilli in resource-limited settings

      Mahmooda Kazmi Adnan Khan Shahana Urooj Kazmi

      Articles Volume 38 Issue 2 June 2013 pp 329-337

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/02/0329-0337

    • Keywords

       

      Bacterial identification; Gram-negative; oxidase-positive; phenotypic; Pseudomonas

    • Abstract

       

      Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology. The diagnosis and surveillance of diseases is dependent, to a great extent, on laboratory services, which cannot function without effective reliable reagents and diagnostics. Despite the advancement in microbiology diagnosis globally, resource-limited countries still struggle to provide an acceptable diagnosis quality which helps in clinical disease management and improve their mortality and morbidity data. During this study an indigenous product, Quick Test Strip (QTS) NE, was developed for the rapid identification of biochemically slower group of Gram-negative oxidase-positive bacilli that covers 19 different bacterial genera. Some of the members belonging to these groups are well-established human pathogens, e.g. various species of Vibrio, Pseudomonas, Burkholderia, Aeromonas, Achromobacter and Stenotrophomonas. This study also evaluates the performance of QTS-NE by comparing with genotypic characterization methods. A total of 232 clinical and reference bacterial isolates were tested by three different methods. QTS-NE provides 100% concordant results with other rapid identification and molecular characterization methods and confirms the potential to be used in clinical diagnosis.

    • Author Affiliations

       

      Mahmooda Kazmi1 Adnan Khan1 Shahana Urooj Kazmi1

      1. Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, University Road, Karachi 75270, Pakistan

    • Dates

       
      Published
      June 2013
      Manuscript received
      9 November 2012
      Accepted
      5 March 2013

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