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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/02/0301-0309

    • Keywords

       

      Anopheles stephensi; cDNA library; germ band retraction; mosquito; transcriptome

    • Abstract

       

      Germ band retraction (GBR) stage is one of the important stages during insect development. It is associated with an extensive epithelial morphogenesis and may also be pivotal in generation of morphological diversity in insects. Despite its importance, only a handful of studies report the transcriptome repertoire of this stage in insects. Here, we report generation, annotation and analysis of ESTs from the embryonic stage (16–22 h post fertilization) of laboratory-reared Anopheles stephensi mosquitoes. A total of 1002 contigs were obtained upon clustering of 1140 high-quality ESTs, which demonstrates an astonishingly low transcript redundancy (12.1%). Putative functions were assigned only to 213 contigs (21%), comprising mainly of transcripts encoding protein synthesis machinery. Approximately 78% of the transcripts remain uncharacterized, illustrating a lack of sequence information about the genes expressed in the embryonic stages of mosquitoes. This study highlights several novel transcripts, which apart from insect development, may significantly contribute to the essential biological complexity underlying insect viability in adverse environments. Nonetheless, the generated sequence information from this work provides a comprehensive resource for genome annotation, microarray development, phylogenetic analysis and other molecular biology applications in entomology.

    • Author Affiliations

       

      Kaustubh Gokhale1 2 Deepak P Patil1 3 Dhiraj P Dhotre1 Rajnikant Dixit1 4 Murlidhar J Mendki5 Milind S Patole1 Yogesh S Shouche1

      1. National Centre for Cell Science, Ganeshkhind, Pune 411 007
      2. School of Life Sciences, Arizona State University, Tempe, AZ USA
      3. Center for RNA Biology, The Ohio State University, Columbus 43202 OH USA
      4. National Institute of Malaria Research, Sector 8, Dwarka, Delhi 110077, India
      5. Entomology Division, Defense Research and Development Establishment, Gwalior 474 002

    • Dates

       
      Published
      June 2013
      Manuscript received
      2 August 2012
      Accepted
      7 March 2013

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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