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    • Keywords


      Anti-repressor; co-repressors; epr; ScoC (Hpr); SinR; Spo0A

    • Abstract


      Bacillus subtilis under nutritional deprivation exhibits several physiological responses such as synthesis of degradative enzymes, motility, competence, sporulation, etc. At the onset of post-exponential phase the global response regulator, Spo0A, directly or indirectly activates the expression of genes involved in the above processes. These genes are repressed during the exponential phase by a group of proteins called transition state regulators, e.g. AbrB, ScoC and SinR. One such post-exponentially expressed gene is epr, which encodes a minor extracellular serine protease and is involved in the swarming motility of B. subtilis. Deletion studies of the upstream region of epr promoter revealed that epr is co-repressed by transition state regulators, SinR and ScoC. Our study shows that Spo0A positively regulates epr expression by nullifying the repressive effect of co-repressors, SinR and ScoC. We demonstrate via in vitro mobility shift assays that Spo0A binds to the upstream region of epr promoter and in turn occludes the binding site of one of the co-repressor, SinR. This explains the mechanism behind the positive regulatory effect of Spo0A on epr expression.

    • Author Affiliations


      Monica Gupta1 2 Madhulika Dixit1 3 K Krishnamurthy Rao1

      1. Gene Regulation Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology (IIT) Bombay, Powai, Mumbai 400 076, India
      2. National Institute of Child Health and Human Development (NICHD), 6 Center Dr. Room 2A09, National Institutes of Health (NIH), Bethesda, MD 20892, USA
      3. Department of Biotechnology, Indian Institute of Technology (IIT) Madras, Chennai 600036, India
    • Dates

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