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    • Bioluminescent bioreporter for assessment of arsenic contamination in water samples of India

      Pratima Sharma Shahzada Asad Arif Ali

      Articles Volume 38 Issue 2 June 2013 pp 251-258

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/02/0251-0258

    • Keywords

       

      ars operon; arsenic; bacterial sensor; bioluminescence; bioreporter

    • Abstract

       

      In the present study the most efficient 𝑅-factor controlling the ars operon was selected after screening of 39 Escherichia coli isolates by minimum inhibitory concentration test (MIC) studies from water samples of different geographical locations of India. Among all, strain isolated from Hooghly River (West Bengal) was found to have maximum tolerance towards arsenic and was further used for the development of bioreporter bacteria. Cloning of the ars regulatory element along with operator-promotor and luxCDABE from Photobacteria into expression vector has been accomplished by following recombinant DNA protocols. The bioreporter sensor system developed in this study can measure the estimated range of 0.74–60 𝜇g of As/L and is both specific and selective for sensing bioavailable As. The constructed bacterial biosensor was further used for the determination of arsenic ion concentration in different environmental samples of India.

    • Author Affiliations

       

      Pratima Sharma1 Shahzada Asad1 Arif Ali1

      1. Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia University, New Delhi 110 025, India

    • Dates

       
      Published
      June 2013
      Manuscript received
      31 August 2012
      Accepted
      28 January 2013

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