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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/038/02/0239-0243

    • Keywords

       

      16S rRNA genes sequencing; bifidobacteria; Elephas maximus; herbivores; identification

    • Abstract

       

      Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60±0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets.

    • Author Affiliations

       

      Vera Bunesova1 Eva Vlkova1 Vojtech Rada1 Jiri Killer1 2 Vladimir Kmet3

      1. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, Prague 6-Suchdol 1652 1, Czech Republic
      2. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4-Krč 14200, Czech Republic
      3. Institute of Animal Physiology of Slovak Academy of Sciences, Soltesovej 4, Kosice 040 01, Slovakia
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    • Supplementary Material

       
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