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    • Keywords


      Cotton; cry1Ac; genomic DNA isolation; high-resolution melting curve analysis; radicle tip; seed purity testing

    • Abstract


      Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing.

    • Author Affiliations


      P Srinivasa Rao1 2 P Sateesh Kumar3 Ramesh V Sonti1

      1. Centre for Cellular and Molecular Biology (CCMB), Uppal Road, Hyderabad 500 007, India
      2. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, India
      3. Nuziveedu Seeds, Road No. 12, Banjara Hills, Hyderabad 500 034, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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