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    • Keywords

       

      Fatty acid acylation; membrane association; model membranes; tyrosine kinases

    • Abstract

       

      The SH4 domain of Src family of nonreceptor protein tyrosine kinases represents the extreme N-terminal 1–16 amino acid region which mediates membrane association of these proteins and facilitates their functions. The SH4 domains among Src members lack well-defined sequence consensus and vary in the net charge. However, they readily anchor to the cytoplasmic face of the plasma membrane upon fatty acid acylation. Here, we report the membrane association of differentially acylated SH4 domain of Lck kinase, which has net negative charge at physiological pH. Our results suggest that despite the net negative charge, the SH4 domain of Lck associates with membranes upon fatty acid acylation. While myristoylation at the N-terminus is sufficient for providing membrane anchorage, multiple acylation determines orientation of the peptide chain with respect to the lipid bilayer. Hence, fatty acylation serves more than just a lipid anchor. It has an important role in regulating the spatial orientation of the peptide domain with respect to the lipid bilayer, which could be important for the interaction of the other domains of these kinases with their partners.

    • Author Affiliations

       

      Anoop Rawat1 Avaronnan Harishchandran1 2 Ramakrishnan Nagaraj1

      1. CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007
      2. Novozymes South Asia Pvt Ltd, Plot No. 32, 47-50, EPIP Area, Whitefield, Bangalore 560 066
    • Dates

       
  • Journal of Biosciences | News

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