• COCHLEATA controls leaf size and secondary inflorescence architecture via negative regulation of UNIFOLIATA (LEAFY ortholog) gene in garden pea Pisum sativum

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      https://www.ias.ac.in/article/fulltext/jbsc/037/06/1041-1059

    • Keywords

       

      Bracteole differentiation; COCHLEATA gene; garden pea; leaf differentiation; LEAFY ortholog; leaf size; Pisum sativum; secondary inflorescence; stub histology; UNIFOLIATA gene; UNIFOLIATA repression

    • Abstract

       

      UNIFOLIATA [(UNI) or UNIFOLIATA-TENDRILLED ACACIA (UNI-TAC)] expression is known to be negatively regulated by COCHLEATA (COCH) in the differentiating stipules and flowers of Pisum sativum. In this study, additional roles of UNI and COCH in P. sativum were investigated. Comparative phenotyping revealed pleiotropic differences between COCH (UNI-TAC and uni-tac) and coch (UNI-TAC and uni-tac) genotypes of common genetic background. Secondary inflorescences were bracteole-less and bracteolated in COCH and coch genotypes, respectively. In comparison to the leaves and corresponding sub-organs and tissues produced on COCH plants, coch plants produced leaves of 1.5-fold higher biomass, 1.5-fold broader petioles and leaflets that were 1.8-fold larger in span and 1.2-fold dorso-ventrally thicker. coch leaflets possessed epidermal cells 1.3-fold larger in number and size, 1.4-fold larger spongy parenchyma cells and primary vascular bundles with 1.2-fold larger diameter . The transcript levels of UNI were at least 2-fold higher in coch leaves and secondary inflorescences than the corresponding COCH organs. It was concluded that COCH negatively regulated UNI in the differentiating leaves and secondary inflorescences and thereby controlled their sizes and/or structures. It was also surmised that COCH and UNI (LFY homolog) occur together widely in stipulate flowering plants.

    • Author Affiliations

       

      Vishakha Sharma1 Swati Chaudhary1 Arvind Kumar1 Sushil Kumar1

      1. Genetical Genomics Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110 067
    • Dates

       
  • Journal of Biosciences | News

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