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    • Keywords


      DNA markers; plants; sequencing

    • Abstract


      Advances in DNA sequencing provide tools for efficient large-scale discovery of markers for use in plants. Discovery options include large-scale amplicon sequencing, transcriptome sequencing, gene-enriched genome sequencing and whole genome sequencing. Examples of each of these approaches and their potential to generate molecular markers for specific applications have been described. Sequencing the whole genome of parents identifies all the poly-morphisms available for analysis in their progeny. Sequencing PCR amplicons of sets of candidate genes from DNA bulks can be used to define the available variation in these genes that might be exploited in a population or germplasm collection. Sequencing of the transcriptomes of genotypes varying for the trait of interest may identify genes with patterns of expression that could explain the phenotypic variation. Sequencing genomic DNA enriched for genes by hybridization with probes for all or some of the known genes simplifies sequencing and analysis of differences in gene sequences between large numbers of genotypes and genes especially when working with complex genomes. Examples of application of the above-mentioned techniques have been described.

    • Author Affiliations


      Robert J Henry1 Mark Edwards2 Daniel L E Waters2 S Gopala Krishnan2 3 Peter Bundock2 Timothy R Sexton4 Ardashir K Masouleh2 Catherine J Nock2 Julie Pattemore5

      1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane QLD 4072, Australia
      2. Southern Cross Plant Science, Southern Cross University, Lismore, NSW 2480, Australia
      3. Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
      4. Departmant of Forest Sciences, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
      5. EH Graham Centre for Agricultural Innovation, School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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