Click here to view fulltext PDF

Permanent link:
https://www.ias.ac.in/article/fulltext/jbsc/037/04/0731-0747

Affymetrix GeneChip; MicroRNAs; Nitrogen; Rice; Transcription profile

Nitrogen is an essential mineral nutrient required for plant growth and development. Insufficient nitrogen (N) supply triggers extensive physiological and biochemical changes in plants. In this study, we used Affymetrix GeneChip rice genome arrays to analyse the dynamics of rice transcriptome under N starvation. N starvation induced or suppressed transcription of 3518 genes, representing 10.88% of the genome. These changes, mostly transient, affected various cellular metabolic pathways, including stress response, primary and secondary metabolism, molecular transport, regulatory process and organismal development. 462 or 13.1% transcripts for N starvation expressed similarly in root and shoot. Comparative analysis between rice and Arabidopsis identified 73 orthologous groups that responded to N starvation, demonstrated the existence of conserved N stress coupling mechanism among plants. Additional analysis of transcription profiles of microRNAs revealed differential expression of miR399 and miR530 under N starvation, suggesting their potential roles in plant nutrient homeostasis.

Hongmei Cai^{1 2} Yongen Lu¹ Weibo Xie¹ Tong Zhu³ Xingming Lian¹

1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Wuhan, China
2. Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
3. Syngenta Biotechnology Inc, 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA

Published

September 2012

Manuscript received

18 March 2011

Accepted

22 June 2012

• Cai.pdf
• Supplemental Table S1.xls
• Supplemental Table S2.xls
• Supplemental Table S3.xls
• Supplemental Table S4.xls
• Supplemental Table S5.xls
• Supplemental Table S6.pdf