• Enantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells in organic solvents

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    • Keywords


      Enantioselective hydrolysis; enantioselectivity; epichlorohydrin; epoxide hydrolase; organic solvent

    • Abstract


      The enantioselective hydrolysis of racemic epichlorohydrin for the production of enantiopure (𝑆)-epichloro-hydrin using whole cells of Aspergillus niger ZJB-09173 in organic solvents was investigated. Cyclohexane was used as the reaction medium based on the excellent enantioselectivity of epoxide hydrolase from A. niger ZJB-09173 in cyclohexane. However, cyclohexane had a negative effect on the stability of epoxide hydrolase from A. niger ZJB-09173. In the cyclohexane medium, substrate inhibition, rather than product inhibition of catalysis, was observed in the hydrolysis of racemic epichlorohydrin using A. niger ZJB-09173. The racemic epichlorohydrin concentration was markedly increased by continuous feeding of substrate without significant decline of the yield. Ultimately, 18.5% of (𝑆)-epichlorohydrin with 98% enantiomeric excess from 153.6 mM of racemic epichlorohydrin was obtained by the dry cells of A. niger ZJB-09173, which was the highest substrate concentration in the production of enantiopure (𝑆)-epichlorohydrin by epoxide hydrolases using an organic solvent medium among the known reports.

    • Author Affiliations


      Huo-Xi Jin1 2 Zhong-Ce Hu1 2 Yu-Guo Zheng1 2

      1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
      2. Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, China
    • Dates

  • Journal of Biosciences | News

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