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      https://www.ias.ac.in/article/fulltext/jbsc/037/04/0635-0645

    • Keywords

       

      Carotenoids; galactosyl-diacyl-glycerolipids; HPLC-MS; phosphatidylglycerol; Synechococcus sp.

    • Abstract

       

      Time course of carotenoid and membrane lipid variation during high light (HL) acclimation (about 85 𝜇mol m−2 s−1), after transfer from low light (LL) (5–10 μmol m−2 s−1), was determined in a marine Synechococcus strain. High-performance liquid chromatography (HPLC) coupled to diode array detector (DAD) or electrospray ionization mass spectrometry (ESI-MS) was used for compound separation and detection. Myxoxanthophyll rose within a time interval of 8 h to 24 h after the onset of exposure to HL. 𝛽-carotene content started to decrease after 4 h of the onset of exposure to HL. Zeaxanthin content rose with exposure to HL, but it was only significant after 24 h of exposure. Carotenoid changes are in agreement with a coordinated activity of the enzymes of the myxoxanthophyll biosynthetic pathway, with no rate-limiting intermediate steps. Lipid analysis showed all species with a C18:3/C16:0 composition increased their content, the changes of PG(18:3/16:0) and MGDG(18:3/16:0) being primarily significant. Major lipid changes were also found to occur within 24 h. These changes might suggest reduction and reorganization of the thylakoid membrane structure. Hypotheses are also drawn on the role played by lipid molecule shape and their possible effect in membrane fluidity and protein accommodation.

    • Author Affiliations

       

      Olimpio Montero1 Alberto Sánchez-Guijo2 Luis M Lubián3 Gonzalo Martínez-Rodríguez3

      1. Centre for Biotechnology Development (CDB), CSIC, Francisco Vallés 8, 47151 Boecillo (Technological Park), Valladolid, Spain
      2. Institute of Molecular Biology and Genetics (IBGM), CSIC-UVA, Sanz y Fores 3, 47003 Valladolid, Spain
      3. Institute for Marine Sciences of Andalusia (ICMAN), CSIC, Av. República Saharaui 2, 11510 Puerto Real, Cádiz, Spain
    • Dates

       
  • Journal of Biosciences | News

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