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    • Keywords


      Chromosome segment duplications; dominant suppressor of RIP; genome defense in fungi

    • Abstract


      Repeat-induced point mutation (RIP) is a sexual stage-specific mutational process of Neurospora crassa and other fungi that alters duplicated DNA sequences. Previous studies from our laboratory showed that chromosome segment duplications (Dps) longer than ∼300 kbp can dominantly suppress RIP, presumably by titration of the RIP machinery, and that although Dps < 200 kbp did not individually suppress RIP, they could do so in homozygous and multiply heterozygous crosses, provided the sum of the duplicated DNA exceeds ∼300 kbp. Here we demonstrate suppression of RIP in a subset of progeny carrying the normally sub-threshold 154 kbp Dp(R2394) from a cross of T(R2394) to the wild isolated Carrefour Mme. Gras strain (CMG). Thus, the CMG strain contains a factor that together with Dp(R2394) produces a synthetic RIP suppressor phenotype. It is possible that the factor is a cryptic Dp that together with Dp(R2394) can exceed the size threshold for titration of the RIP machinery and thereby causes RIP suppression.

    • Author Affiliations


      Mukund Ramakrishnan1 T Naga Sowjanya1 Kranthi B Raj1 Durgadas P Kasbekar1

      1. Centre for Cellular and Molecular Biology (CSIR), Hyderabad 500 007, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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