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      https://www.ias.ac.in/article/fulltext/jbsc/036/04/0659-0668

    • Keywords

       

      CIB1; 2-DE; hepatocellular carcinoma; proteomics; proliferation

    • Abstract

       

      Hepatocellular carcinoma (HCC), among the most common malignancies worldwide, remains a major threat to public health, and there is an urgent need to identify novel biomarkers for diagnosis, prognosis and targets for anti-cancer treatment. In this study, two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis was used to identify differentially expressed proteins among the HCC tumour centre, tumour margin and nontumourous liver tissues. In total, 52 spots with significant alteration were positively identified by MS/MSanalysis. Altered expression of representative proteins, including CIB1, was validated by Western blotting. Immunostaining suggested an increase tendency of CIB1 expression from nontumourous liver tissue to tumour centre. Knockdown of CIB1 expression by RNA interference led to the significant suppression of the cell growth in hepatoma HepG2 cells. These data suggest that CIB1 may be used as a novel prognostic factor and possibly an attractive therapeutic target for HCC.

    • Author Affiliations

       

      Tong Junrong1 Zhou Huancheng2 H E Feng1 Gao Yi2 Yang Xiaoqin3 Luo Zhengmao1 Zhang Hong1 Zeng Jianying1 Wang Yin1 Huang Yuanhang1 Zhang Jianlin1 Sun Longhua1 He Guolin2

      1. Department of Nephrology, Guangzhou Army General Hospital Guangzhou 510010, China
      2. Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University 510282, China
      3. Institute of Genetic Engineering, Southern Medical University 510515, China
    • Dates

       
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