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https://www.ias.ac.in/article/fulltext/jbsc/036/04/0659-0668

CIB1; 2-DE; hepatocellular carcinoma; proteomics; proliferation

Hepatocellular carcinoma (HCC), among the most common malignancies worldwide, remains a major threat to public health, and there is an urgent need to identify novel biomarkers for diagnosis, prognosis and targets for anti-cancer treatment. In this study, two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis was used to identify differentially expressed proteins among the HCC tumour centre, tumour margin and nontumourous liver tissues. In total, 52 spots with significant alteration were positively identified by MS/MSanalysis. Altered expression of representative proteins, including CIB1, was validated by Western blotting. Immunostaining suggested an increase tendency of CIB1 expression from nontumourous liver tissue to tumour centre. Knockdown of CIB1 expression by RNA interference led to the significant suppression of the cell growth in hepatoma HepG2 cells. These data suggest that CIB1 may be used as a novel prognostic factor and possibly an attractive therapeutic target for HCC.

Tong Junrong¹ Zhou Huancheng² H E Feng¹ Gao Yi² Yang Xiaoqin³ Luo Zhengmao¹ Zhang Hong¹ Zeng Jianying¹ Wang Yin¹ Huang Yuanhang¹ Zhang Jianlin¹ Sun Longhua¹ He Guolin²

1. Department of Nephrology, Guangzhou Army General Hospital Guangzhou 510010, China
2. Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University 510282, China
3. Institute of Genetic Engineering, Southern Medical University 510515, China

Published

September 2011

Manuscript received

28 February 2011

Accepted

30 May 2011

Early published

16 August 2011

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