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      https://www.ias.ac.in/article/fulltext/jbsc/035/04/0557-0564

    • Keywords

       

      Colon cancer; 2-DE; drug resistance; irinotecan; proteomics

    • Abstract

       

      Chemotherapeutic drug resistance is a frequent cause of treatment failure in colon cancer patients. Several mechanisms have been implicated in drug resistance. However, they are not sufficient to exhaustively account for this resistance emergence. In this study, two-dimensional gel electrophoresis (2-DE) and the PDQuest software analysis were applied to compare the differential expression of irinotecan-resistance-associated protein in human colon adenocarcinoma LoVo cells and irinotecan-resistant LoVo cells (LoVo/irinotecan). The differential protein dots were excised and analysed by ESI-Q-TOF mass spectrometry (MS). Fifteen proteins were identified, including eight proteins with decreased expression and seven proteins with increased expression. The identified known proteins included those that function in diverse biological processes such as cellular transcription, cell apoptosis, electron transport/redox regulation, cell proliferation/differentiation and retinol metabolism pathways. Identification of such proteins could allow improved understanding of the mechanisms leading to the acquisition of chemoresistance.

    • Author Affiliations

       

      Xing-Chen Peng1 Feng-Ming Gong1 Meng Wei1 X I Chen1 Y E Chen1 K E Cheng1 Feng Gao1 Feng Xu1 FENG Bi1 Ji-Yan Liu1

      1. Department of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, No. 37, Guo Xue Xiang, Chengdu 610041, Sichuan Province, China
    • Dates

       
  • Journal of Biosciences | News

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