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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/035/03/0371-0378

    • Keywords

       

      Apoptosis; cis-(dichloro)tetrammineruthenium(III) chloride; cell cycle; DNA damage; S-180

    • Abstract

       

      Ruthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological properties. Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic effects on murine sarcoma 180 (S-180) cells. In an effort to understand the mechanism responsible for their cytotoxicity, study we investigated the genotoxicity, cell cycle distribution and induction of apoptosis caused by cis-(dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride treatment induced significant DNA damage in S-180 cells, as detected by the alkaline comet assay. In the cell cycle analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase, accompanied by a decrease in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent increase in the sub-G1 phase (indicating apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases. In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner, as observed by the increased numbers of annexin V-positive cells. Taken together, these findings strongly demonstrate that DNA damage, cell cycle changes and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetrammineruthenium(III) chloride on S-180 cells.

    • Author Affiliations

       

      Aliny Pereira De Lima1 Flávia De Castro Pereira1 Cesar Augusto Sam Tiago Vilanova-Costa1 Alessandra De Santana Braga Barbosa Ribeiro1 Luiz Alfredo Pavanin2 Wagner Batista Dos Santos3 Elisângela De Paula Silveira-Lacerda1

      1. Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Goiânia, Goiás, Brazil
      2. Instituto de Química, Universidade Federal de Uberlândia - UFU, Uberlândia, Minas Gerais, Brazil
      3. Faculdade Patos de Minas - FPM, Patos de Minas, Minas Gerais, Brazil
    • Dates

       
  • Journal of Biosciences | News

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