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    • Keywords


      Apoptosis; cationic liposome; human lung adenocarcinomas; survivin

    • Abstract


      Anti-apoptosis plays an important role in tumour formation and development. Survivin is a member of the inhibitor of apoptosis (IAP) family, which is a target for anti-cancer drug exploitation was replaced as development. We investigated the role of the homo dominant-negative mutant Survivin-T34A in suppressing human lung adenocarcinomas (A549). The anti-tumour activity of HSurvivinT34A plasmid was evaluated in the A549 cell line and nude mice bearing A549 subcutaneous tumours. Low-dose systemic administration was continuously used. The HSurvivinT34A plasmid (5 𝜇g/one) complexed with a cationic liposome (DOTAP/Chol) significantly inhibited tumour growth in our model. We observed microvessel density degradation by CD31 immunohistochemistry and apoptotic cell increase by TUNEL assay, PI staining and flow cytometric analysis in the treated group. The present findings suggest that the HSurvivinT34A plasmid complexed with a cationic liposome may provide an effective approach to inhibit the growth of human lung adenocarcinomas in vitro and in vivo.

    • Author Affiliations


      Yan Shan1 Chunting Wang1 Li Yang1 Li Juan Chen1 Hong Xin Deng1 Han Shuo Yang1 Zhimian Li1 Zhiyong Li1 Li Pan1 Fei Leng1 Yuquan Wei1

      1. State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Chengdu, Sichuan, 610041, China
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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