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      https://www.ias.ac.in/article/fulltext/jbsc/034/06/0899-0908

    • Keywords

       

      2D gel electrophoresis; human seminal plasma; heparin-binding proteins; MALDI-TOF; sperm capacitation

    • Abstract

       

      Glycosaminoglycans, especially heparin, are involved in various cell processes such as apoptosis, cell cycle control, platelet activation, capacitation, acrosome reaction and sperm decondensation. Heparin-binding proteins (HBPs) are essential constituents of human seminal fluid, which bind to sperm lipids containing the phosphorylcholine group and mediate the fertilization process. We utilized a proteomic set-up consisting of affinity chromatography, isoelectric focusing (IEF) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI TOF/MS) for protein analysis of human HBPs. We resolved 70 different spots on two-dimensional (2-D) gel and subsequently identified these proteins. Forty different types of proteins were identified. Functional analysis revealed that 38% of the proteins belonged to the enzyme category, 20% were involved in RNA processing and transcription, 18% in structure and transport function, and 16% in cell recognition and signal transduction. We also identified 8% of proteins with unknown functions, although their expression in seminal fluid has been documented. Proteins of seminal fluid that bind heparin may be directly involved in sperm capacitation and acrosome reaction (AR), which are the two critical steps for fertilization. This information on HBPs would be useful for identifying potential biomarkers of fertility in the near future.

    • Author Affiliations

       

      Vijay Kumar1 Md Imtaiyaz Hassan1 Anil Kumar Tomar1 Tara Kashav2 Jaya Nautiyal1 Sarman Singh3 Tej P Singh1 Savita Yadav1

      1. Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India
      2. School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
      3. Department of Lab Medicine, All India Institute of Medical Sciences, New Delhi 110 029, India
    • Dates

       
  • Journal of Biosciences | News

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