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    • Keywords


      Apoptosis; GalNAc-T14; IGFBP-3; RNAi

    • Abstract


      Insulin-like growth factor binding protein-3 (IGFBP-3) is known to induce apoptosis in an insulin-like growth factor (IGF)-dependent and IGF-independent manner, but the mechanism underlying the IGF-independent effects remains unclear. Polypeptide 𝑁-acetylgalactosaminyltransferase 14 (GalNAc-T14) is a novel IGFBP-3 binding partner. In this paper, small interference RNA (siRNA) targeting GalNAc-T14 was used to examine whether GalNAc-T14 affects the apoptotic action of IGFBP-3. Using semi-quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) and western blot analysis, we determined that GalNAc-T14 expression was downregulated by the siRNA directed against GalNAc-T14. Apoptosis analysis of IGFBP-3-overexpressing cells treated with siRNA against GalNAc-T14 was performed to determine if GalNAc-T14 was specifically involved in IGFBP-3 signalling. The results, as determined by flow cytometric analysis and caspase-3 assay, showed that the extent of apoptosis induced by IGFBP-3 increased with RNA interference (RNAi) knockdown of GalNAc-T14. Our data suggest that GalNAc-T14 influences the apoptotic action of IGFBP-3 and might mediate the signalling pathway of IGFBP-3. Experiments to determine the role of GalNAc-T14 in the regulation of apoptosis induced by IGFBP-3 are under way.

    • Author Affiliations


      Chen Wu1 Yaojun Shan1 Xinxia Liu2 Wenqian Song1 Jiali Wang3 Minji Zou4 Min Wang4 Donggang Xu4

      1. College of Life Sciences, Hebei University, Baoding, Hebei, 071002, P R China
      2. Health Sciences Centre, Hebei University, Baoding, Hebei, 071002, P R China
      3. College of Pharmaceutical Sciences, Hebei University, Baoding, Hebei, 071002, P R China
      4. Institute of Basic Medical Sciences, Beijing 100850, P R China
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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