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      https://www.ias.ac.in/article/fulltext/jbsc/034/02/0199-0202

    • Keywords

       

      Agrobacterium tumefaciens; insertion sequence; transgene inactivation; transposition

    • Abstract

       

      Agrobacterium strains harbour insertion sequences, which are known to transpose into genomes as well as into Ti plasmids. In this study we report the inactivation of a transgene due to transposition of the A. tumefaciens insertion sequence IS136. The transposition was discovered following transformation of plant tissues, although the fidelity of the binary vector was confirmed following transformation into Agrobacterium. Such transpositions are rare but can occur and it is thus important to check the fidelity of the binary vector at different times of Agrobacterium growth in order to avoid failure in achieving transgene expression.

    • Author Affiliations

       

      Preeti Rawat1 Sanjeev Kumar1 2 Deepak Pental1 3 Pradeep Kumar Burma1

      1. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
      2. Amity Institute of Biotechnology, Amity University, Noida 201303, India
      3. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India

    • Dates

       
      Published
      June 2009
      Manuscript received
      1 October 2008
      Accepted
      6 April 2009
      Early published
      29 April 2009

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