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Agrobacterium tumefaciens; insertion sequence; transgene inactivation; transposition

Agrobacterium strains harbour insertion sequences, which are known to transpose into genomes as well as into Ti plasmids. In this study we report the inactivation of a transgene due to transposition of the A. tumefaciens insertion sequence IS136. The transposition was discovered following transformation of plant tissues, although the fidelity of the binary vector was confirmed following transformation into Agrobacterium. Such transpositions are rare but can occur and it is thus important to check the fidelity of the binary vector at different times of Agrobacterium growth in order to avoid failure in achieving transgene expression.

Preeti Rawat¹ Sanjeev Kumar^{1 2} Deepak Pental^{1 3} Pradeep Kumar Burma¹

1. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
2. Amity Institute of Biotechnology, Amity University, Noida 201303, India
3. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India

Published

June 2009

Manuscript received

1 October 2008

Accepted

6 April 2009

Early published

29 April 2009