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      https://www.ias.ac.in/article/fulltext/jbsc/034/01/0021-0026

    • Keywords

       

      Aliphatic nitriles; biotransformation; immobilization; nitrilase; Streptomyces; thermophilic

    • Abstract

       

      The biotransformation of acrylonitrile was investigated using thermophilic nitrilase produced from a new isolate Streptomyces sp. MTCC 7546 in both the free and immobilized state. Under optimal conditions, the enzyme converts nitriles to acids without the formation of amides. The whole cells of the isolate were immobilized in agar-agar and the beads so formed were evaluated for 25 cycles at 50°C. The enzyme showed a little loss of activity during reuse. Seventy-one per cent of 0.5 M acrylonitrile was converted to acid at 6 h of incubation at a very low density of immobilized cells, while 100% conversion was observed at 3 h by free cells.

    • Author Affiliations

       

      V K Nigam1 A K Khandelwal1 R K Gothwal1 M K Mohan1 B Choudhury2 A S Vidyarthi3 P Ghosh1

      1. Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
      2. Faculty of Biological Science, Indian Institute of Technology, Roorkee 247 667, India
      3. Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
    • Dates

       
  • Journal of Biosciences | News

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