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    • Keywords


      Bauhinia variegata; Caesalpinoideae; lectin

    • Abstract


      A new galactose-specific lectin was purified from seeds of a Caesalpinoideae plant, Bauhinia variegata, by affinity chromatography on lactose–agarose. Protein extracts haemagglutinated rabbit and human erythrocytes (native and treated with proteolytic enzymes), showing preference for rabbit blood treated with papain and trypsin. Among various carbohydrates tested, the lectin was best inhibited by D-galactose and its derivatives, especially lactose. SDS-PAGE showed that the lectin, named BVL, has a pattern similar to other lectins isolated from the same genus, Bauhinia purpurea agglutinin (BPA). The molecular mass of BVL subunit is 32 871 Da, determined by MALDI-TOF spectrometry. DNA extracted from B. variegata young leaves and primers designed according to the B. purpurea lectin were used to generate specific fragments which were cloned and sequenced, revealing two distinct isoforms. The bvl gene sequence comprised an open reading frame of 876 base pairs which encodes a protein of 291 amino acids. The protein carried a putative signal peptide. The mature protein was predicted to have 263 amino acid residues and 28 963 Da in size.

    • Author Affiliations


      Luciano S Pinto1 Celso S Nagano2 Taianá M Oliveira2 Tales R Moura2 Alexandre H Sampaio3 Henri Debray4 Vicente P Pinto5 Odir A Dellagostin1 Benildo S Cavada2

      1. Centro de Biotecnologia, Universidade Federal de Pelotas, Campus Universitário, s/no · 96010-900. Pelotas, RS, Brazil
      2. Laboratório de Moléculas Biologicamente Ativas - Biomol-lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil
      3. Departamento de Engenharia da Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brazil
      4. Laboratoire de Chimie Biologique et Unite Mixte de Recherche, Centre National de la Recherche Scientifique (UMR CNRS). 8576, Université des Sciences et Technologies de Lille (USTL), Villeneuve d'Ascq, France
      5. Faculdade de Medicina de Sobral, Universidade Federal do Ceará, Sobral, CE, Brazil
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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