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      https://www.ias.ac.in/article/fulltext/jbsc/033/02/0279-0287

    • Keywords

       

      Biosensor; functional membrane; horseradish peroxidase; nanoparticles

    • Abstract

       

      A novel nafion–riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of –562 ± 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin–nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 ± 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 𝜇M.

    • Author Affiliations

       

      S Rezaei-Zarchi1 2 A A Saboury1 A Javed3 A Barzegar1 S Ahmadiam1 A Bayandori-Moghaddam4

      1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
      2. Department of Biology, Payam-e-Noor University, Yazd, Iran
      3. Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
      4. Department of Chemistry, Faculty of Sciences, University of Tehran, Tehran, Iran
    • Dates

       
  • Journal of Biosciences | News

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