• Immunocytochemical detection of glucagon and insulin cells in endocrine pancreas and cyclic disparity of plasma glucose in the turtle Melanochelys trijuga

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/033/02/0239-0247

    • Keywords

       

      Avidin–biotin peroxidase labelling; glucagon-IR; insulin-IR; plasma glucose; seasonal variation; turtle

    • Abstract

       

      The present investigation was carried out to know the seasonal variation in plasma glucose, insulin and glucagon cells during the reproductive cycle of untreated Melanochelys trijuga. Pancreatic endocrine cells were immunochemically localized. Insulin-immunoreactive (IR) cells occurred in groups of 3–20 and were in close apposition, while glucagon-IR cells were distributed individually between the exocrine pancreas or formed anastomosing cords where cells were not intimately attached. Whenever both IR cell types were present together forming an islet, insulin-IR cells formed clusters in the centre with glucagon-IR cells being scattered at the periphery. Glucagon-IR cells seemed to be secretory throughout the pancreas during the reproductive cycle, while insulin-IR cells were found to be pulsating in their secretion. Mean size of the islet was 1.306, 0.184 and 2.558 mm in the regenerative, reproductive and regressive periods, respectively. In general, insulin-IR cells measured 5.18 𝜇m and glucagon-IR cells 5.22 𝜇m in their longest axis. Invariably, glucagon-IR cells were more in number than insulin-IR cells. The fasting plasma glucose level was 69.97 mg% during the regenerative period, which increased to 97.96 mg% during the reproductive period, and reached a peak value of 113.52 mg% in the regressive period.

    • Author Affiliations

       

      Vidya R Chandavar1 2 Prakash R Naik1

      1. Department of Studies in Zoology, University of Mysore, Manasagangorti, Mysore 570 006, India
      2. Yuvaraja's College, University of Mysore, Mysore 570 005, India
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.