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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/033/02/0221-0230

    • Keywords

       

      Cell wall; Mycobacterium tuberculosis; phospholipase C

    • Abstract

       

      Mycobacterium tuberculosis survives and persists for prolonged periods within its host in an asymptomatic, latent state and can reactivate years later if the host’s immune system weakens. The dormant bacilli synthesize and accumulate triacylglycerol, reputed to be an energy source during latency. Among the phospholipases, phospholipase C plays an important role in the pathogenesis. Mutations in a known phospholipase C, plcC, of M. tuberculosis attenuate its growth during the late phase of infection in mice. Hydrolysis of phospholipids by phospholipase C generates diacylglycerol, a well-known signalling molecule that participates in the activation of extracellular signal-regulated kinases (ERK) through protein kinase C leading to macrophage activation. In the present study, we show that M. tuberculosis possesses an additional cell wall-associated protein, Rv3487c, with phospholipase C activity. The recombinant Rv3487c hydrolyses the substrate phosphatidylcholine and generates diacylglycerol by removing the phosphocholine. Furthermore, Rv3487c is expressed during infection as it exhibits significant humoral immunoreactivity with sera from children with tuberculosis, but not with that from adult patients.

    • Author Affiliations

       

      Madduri Srinivas1 Sona Rajakumari2 Yeddula Narayana1 Beenu Joshi3 V M Katoch3 Ram Rajasekharan2 Kithiganahalli N Balaji1

      1. Department of Microbiology and Cell Biology, Bangalore 560 012, India
      2. Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India
      3. National Jalma Institute of Leprosy and other Mycobacterial Diseases, Tajganj, Agra 282 001, India

    • Dates

       
      Published
      June 2008
      Manuscript received
      20 September 2007
      Accepted
      29 January 2008
      Early published
      28 March 2008

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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