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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/033/02/0185-0193

    • Keywords

       

      Crop biotechnology; enhancer traps; nematode resistance; root expression; tissue-specific

    • Abstract

       

      A set of Ds-element enhancer trap lines of Arabidopsis thaliana was generated and screened for expression patterns leading to the identification of a line that showed root-specific expression of the bacterial uidA reporter gene encoding 𝛽-glucuronidase (GUS). The insertion of the Ds element was found to be immediately downstream to a glycosyltransferase gene At1g73160. Analysis of At1g73160 expression showed that it is highly root-specific. Isolation and characterization of the upstream region of the At1g73160 gene led to the definition of a 218 bp fragment that is sufficient to confer root-specific expression. Sequence analysis revealed that several regulatory elements were implicated in expression in root tissue. The promoter identified and characterized in this study has the potential to be applied in crop biotechnology for directing the root-specific expression of transgenes.

    • Author Affiliations

       

      Virupapuram Vijaybhaskar1 Veeraputhiran Subbiah1 Jagreet Kaur1 Pagadala Vijayakumari1 Imran Siddiqi1

      1. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India
    • Dates

       
  • Journal of Biosciences | News

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