1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 32
    5. Issue 6

    • Age-dependent increase in green autofluorescence of blood erythrocytes

      Sanjay Khandelwal Rajiv K Saxena

      Articles Volume 32 Issue 6 September 2007 pp 1139-1145

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/032/06/1139-1145

    • Keywords

       

      Biotin; green autofluorescence; in vivo biotinylation; macrophage depletion; murine erythrocyte aging

    • Abstract

       

      Green auto-fluorescence (GAF) of different age groups of mouse blood erythrocytes was determined by using a double in vivo biotinylation (DIB) technique that enables delineation of circulating erythrocytes of different age groups. A significant increase in GAF was seen for erythrocytes of old age group (age in circulation > 40 days) as compared to young erythrocytes (age < 15 days). Erythrocytes are removed from blood circulation by macrophages in the reticulo-endothelial system and depletion of macrophages results in an increased proportion of aged erythrocytes in the blood. When mice were depleted of macrophages for 7 days by administration of clodronate loaded liposomes, the overall GAF of erythrocytes increased significantly and this increase could be ascribed to an increase in GAF of the oldest population of erythrocytes. Using the DIB technique, the GAF of a cohort of blood erythrocyte generated during a 5 day window was tracked in vivo. GAF of the defined cohort of erythrocytes remained low till 40 days of age in circulation and then increased steeply till the end of the life span of erythrocytes. Taken together our results provide evidence for an age dependent increase in the GAF of blood erythrocytes that is accentuated by depletion of macrophages. Kinetics of changes in GAF of circulating erythrocytes with age has also been defined.

    • Author Affiliations

       

      Sanjay Khandelwal1 Rajiv K Saxena1

      1. School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India

    • Dates

       
      Published
      September 2007
      Manuscript received
      18 April 2007
      Accepted
      21 July 2007
      Early published
      17 August 2007

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2023-2024 Indian Academy of Sciences, Bengaluru.

Contact | Site index