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      https://www.ias.ac.in/article/fulltext/jbsc/032/04/0705-0712

    • Keywords

       

      Araneus ventricosus; baculovirus expression vector; cDNA cloning; flagelliform silk protein; spider.

    • Abstract

       

      A cDNA coding for the C-terminus of spider flagelliform silk protein (AvFlag) was cloned from Araneus ventricosus. Analysis of the cDNA sequence shows that the C-terminus of AvFlag consists of 167 amino acids of a repetitive region and 87 amino acids of a C-terminal non-repetitive region. The peptide motifs found in spider flagelliform silk proteins, GPGGX and GGX, were conserved in the repetitive region of AvFlag. Phylogenetic analysis further confirmed that AvFlag belongs to the spider flagelliform silk proteins. The AvFlag cDNA was expressed as a 28 kDa polypeptide in baculovirus-infected insect cells. As a new expression approach for spider silk protein, the combination of polyhedrin and AvFlag creates a polyhedrin AvFlag fusion protein (61 kDa) that is produced as recombinant polyhedra; this provides a basis for the source of spider silk proteins for various applications.

    • Author Affiliations

       

      Kwang Sik Lee1 Bo Yeon Kim1 Yeon Ho Je2 Soo Dong Woo3 Hung Dae Sohn1 Byung Rae Jin1

      1. College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea
      2. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea
      3. Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea
    • Dates

       
  • Journal of Biosciences | News

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