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      https://www.ias.ac.in/article/fulltext/jbsc/032/04/0663-0669

    • Keywords

       

      CymMV; phylogenetic; potexviruses; recombination

    • Abstract

       

      The complete nucleotide sequence of an Indian strain of Cymbidium mosaic virus (CymMV) was determined and compared with other potexviruses. Phylogenetic analyses on the basis of RNA-dependent RNA polymerase (RdRp), triple gene block protein and coat protein (CP) amino acid sequences revealed that CymMV is closely related to the Narcissus mosaic virus (NMV), Scallion virus X (SVX), Pepino mosaic virus (PepMV) and Potato aucuba mosaic virus (PAMV). Different sets of primers were used for the amplification of different regions of the genome through RT-PCR and the amplified genes were cloned in a suitable vector. The full genome of the Indian isolate of CymMV from Phaius tankervilliae shares 96–97% similarity with isolates reported from other countries. It was found that the CP gene of CymMV shares a high similarity with each other and other potexviruses. One of the Indian isolates seems to be a recombinant formed by the intermolecular recombination of two other CymMV isolates. The phylogenetic analyses, Recombination Detection Program (RDP2) analyses and sequence alignment survey provided evidence for the occurrence of a recombination between an Indian isolate (AM055720) as the major parent, and a Korean type-2 isolate (AF016914) as the minor parent. Recombination was also observed between a Singapore isolate (U62963) as the major parent, and a Taiwan CymMV (AY571289) as the minor parent.

    • Author Affiliations

       

      Ang Rinzing Sherpa1 Vipin Hallan1 Promila Pathak2 Aijaz Asghar Zaidi1

      1. Plant Virology Lab, Floriculture Division, Institute of Himalayan Bioresource Technology (CSIR), Post Box # 6, Palampur 176 061, India
      2. Botany Department, Panjab University, Chandigarh 160 014, Indi
    • Dates

       
  • Journal of Biosciences | News

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